p stat1  (Cell Signaling Technology Inc)

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Therapeutic effect of eravacycline against carbapenem-resistant hypervirulent Klebsiella pneumoniae in mouse models

doi: 10.1128/aac.01237-25

Figure Lengend Snippet: Expression and activation of STAT1 in mouse lung tissues. ( A ) mRNA expression levels of STAT1 in the lung tissues of mice across the uninfected control, the infection control, and the treatment groups. ( B ) Protein expression levels of p-STAT1 in the uninfected control, the infection control, and the treatment groups of mice. Data are shown as mean ± standard error of the mean, n = 3. Hashtags (#) represent the infection control group compared to the uninfected control group, and asterisks (*) represent the infection control group compared to the corresponding treatment group (* P < 0.05, ** P < 0.01, and ### P < 0.001).

Article Snippet: The membrane was blocked with TBST buffer (G2150, Servicebio Technology Co., Ltd. Wuhan, China) containing 5% bovine serum albumin and then incubated with primary antibody STAT1 (1:1,000, #9172T, Cell Signaling Technology, MA, USA), p-STAT1 (1:1,000, #7649T, Cell Signaling Technology, MA, USA), and β-actin (1:10,000, AC004, Abclonal, Wuhan, China) overnight at 4°C.

Techniques: Expressing, Activation Assay, Control, Infection

Journal: NPJ Breast Cancer

Article Title: JAK/STAT1-interferon-ISGylation networks in breast cancer resistance to inhibitors of FOXM1 and CDK4/6

doi: 10.1038/s41523-026-00911-6

Figure Lengend Snippet: A Western protein immunoblots of cell extracts from 73 R, PalboR, and AbemaR cells in their basal condition (Veh treatment) or after 24 h treatment with IFNγ (50 ng/ml), or ruxolitinib (rux, 5 µM) or IFNγ plus rux. Levels corrected for loading control β-actin are shown with the WT Veh level set at 1.0. B In situ cell immunofluorescence for ISG15, STAT1, and ERα in parental WT and resistant cells. For each cell type, immunofluorescence intensities of all cells in 3 fields were individually measured and are shown, along with mean ± SEM. Scale bar is 20 microns. CTCF, corrected total cell fluorescence; CNF, corrected nuclear fluorescence. Statistics are one-way ANOVA followed by Fishers LSD multiple comparisons post hoc test. C Expression of STAT1 and several IFN signature genes ( ISG15, IFI27, IFI44, IFIT1, IFIT3 ) in WT, 73 R, PalboR, and Abema R cells. Cells were treated with vehicle, 50 ng/ml IFNγ, 5 μM rux, or IFNγ plus rux for 24 h. RNA was then extracted from cells and gene expression was monitored by qPCR. Triplicate determinations are shown as dots ± SD. Statistical analysis used the Kruskal-Wallis test B and one-way ANOVA followed by Fisher’s LSD Multiple Comparisons post hoc test C with p-values shown above the brackets. *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001. WT Veh is set at 1.

Article Snippet: Primary antibodies were diluted 1:1000, and those purchased from Cell Signaling Technology (RRID:SCR_002071, Danvers, MA) include ERα (RRID:AB_2617128), STAT1 (RRID:AB_2737027), p-STAT1-Tyr701 (RRID:AB_561284), pSTAT1-Ser727 (RRID:AB_2773718), STAT3 (RRID:AB_331757), and ISG15 (RRID:AB_2126201).

Techniques: Western Blot, Control, In Situ, Immunofluorescence, Fluorescence, Expressing, Gene Expression

Journal: NPJ Breast Cancer

Article Title: JAK/STAT1-interferon-ISGylation networks in breast cancer resistance to inhibitors of FOXM1 and CDK4/6

doi: 10.1038/s41523-026-00911-6

Figure Lengend Snippet: A Western blots showing free ISG15 protein and ISGylated proteins in these cells. Cells were treated with control vehicle or 50 ng/ml IFNγ for 24 h. Protein extracts were then prepared and examined for ISGylation on Western blots with antibody that detects ISG15 and ISGylated proteins. Protein bands A-E are highlighted. β-actin in the same cell samples was monitored on a separate Western blot and this is shown at the bottom of the figure. Molecular weight markers (in kDa) are shown in the left-most lane. B STAT1 is an ISGylated protein in 73 R, PalboR, and AbemaR cells. Cell extracts were incubated with IgG control antibody or ISG15 antibody and the immunoprecipitated proteins were probed with a STAT1 antibody in the eluate and boiled bead extracts by Western blot. STAT1α (91 kDa) and STAT1β (84 kDa) are observed. Independent immunoprecipitation experiments were conducted twice for all cells. C Primers specific for STAT1α and STAT1β were used to detect these RNAs by qPCR in WT and resistant cells. Cells were incubated with Veh or 50 ng/ml IFNγ for 24 h prior to RNA analysis. Two independent experiments were conducted, and individual triplicate determinations are shown as dots ± SD. Statistical analysis by one-way ANOVA followed by Fisher’s LSD Multiple Comparisons post hoc test with p-values shown above the brackets. *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001. WT Veh is set at 1.

Article Snippet: Primary antibodies were diluted 1:1000, and those purchased from Cell Signaling Technology (RRID:SCR_002071, Danvers, MA) include ERα (RRID:AB_2617128), STAT1 (RRID:AB_2737027), p-STAT1-Tyr701 (RRID:AB_561284), pSTAT1-Ser727 (RRID:AB_2773718), STAT3 (RRID:AB_331757), and ISG15 (RRID:AB_2126201).

Techniques: Western Blot, Control, Molecular Weight, Incubation, Immunoprecipitation